rabbit anti-fgf7  (Santa Cruz Biotechnology)

Journal: Breast cancer research and treatment

Article Title: Breast Cancer, Diabetes Mellitus and Glucagon-Like Peptide-1 Receptor Toward Exploring Their Possible Associations.

doi: 10.1007/s10549-021-06288-3

Figure Lengend Snippet: Fig. 1 Representative illustra- tion of immunochemical find- ings in breast carcinoma cases examined in this study. 0, 1+, 2+ and 3+ correspond to the intensity score of immunoreac- tivities obtained. The positive controls were pancreatic islets of Langerhans with positive expression of GLP-1R (a), blad- der cancer tumor with that of FGF7 (b) and lung cancer tumor with that of FGFR2 (c)

Article Snippet: A mouse monoclonal antibody against GLP-1R (ab166987, abcam, Cambridge UK), a rabbit polyclonal antibody against FGF7 (HPA043605, Atlas Antibodies, Stockholm Sweden) and a mouse monoclonal antibody against FGFR2 (ab58201, abcam, Cambridge UK) were used as primary antibodies in this study as summarized in Table 2.

Techniques: Expressing

Journal: Polymers

Article Title: Efficient Surface Immobilization of Chemically Modified Hyaluronans for Enhanced Bioactivity and Survival of In Vitro-Cultured Embryonic Salivary Gland Mesenchymal Cells

doi: 10.3390/polym13081216

Figure Lengend Snippet: ( a ) Bright-field images of E13 eSMGs cultured for 48 h in control, solubilized HA–CA (HA–CA (Sol) ), HA–CA-coated surfaces (HA–CA (Co) ), and hyaluronidase-treated HA–CA-coated surfaces (HA–CA (Co/HAD) ). Scale bar = 200 µm ( n = 4). ( b ) Bud number fold changes (48 h/0 h) of eSMGs cultured in each group ( n = 4). ( c – e ) mRNA expression level of ( c ) EGF, ( d ) FGF7, and ( e ) FGF10 in eSMGs cultured for 48 h under each condition ( n = 3). ( f ) Immunofluorescence images of mesenchymal EGF (green) and FGF7 (red) expression in eSMGs cultured for 48 h on control and HA–CA-coated surface. DAPI (blue). Scale bar = 50 µm ( n = 3). Quantification of mesenchymal ( g ) EGF and ( h ) FGF7 expression based on the immunofluorescence images. Control (gray) and HA–CA (Co) (red) ( n = 4). Data are expressed as average ± SEM ( b–h). ** p < 0.01, **** p < 0.001, ns = non-significant ( p > 0.05) by unpaired t -test ( g,h) and one-way ANOVA with Dunnett’s test ( b–e).

Article Snippet: The permeabilized eSMGs or mesenchymal cells were then blocked by immersion in PBSX containing 10% normal donkey serum (NDS; Sigma-Aldrich) and 1% mouse-on-mouse (MOM) blocking reagent (Vector Laboratories, MKB-2213-1; Burlingame, CA, USA) at RT for 3 h. After the blocking step, eSMGs or mesenchymal cells were incubated with PBSX containing primary antibodies (1:100) and 3% NDS at 4 °C for 12 h. The primary antibodies used in the procedure were as follows: goat polyclonal anti-c-kit antibody (R&D System, AF1356; Minneapolis, MN, USA), rat monoclonal anti-CD44 antibody (Abcam, ab157107; Cambridge, UK), mouse monoclonal anti-TUJ1 (R&D System, MAB1195), rabbit polyclonal anti-E-cadherin (Cell Signaling Technology, 3195S; Beverly, MA, USA), mouse monoclonal anti-EGF (Invitrogen, MA5-15606), and rabbit polyclonal anti-FGF7 (Invitrogen, PA5-49715; Carlsbad, CA, USA).

Techniques: Cell Culture, Expressing, Immunofluorescence

Journal: Polymers

Article Title: Efficient Surface Immobilization of Chemically Modified Hyaluronans for Enhanced Bioactivity and Survival of In Vitro-Cultured Embryonic Salivary Gland Mesenchymal Cells

doi: 10.3390/polym13081216

Figure Lengend Snippet: ( a ) Schematic diagram of embryonic salivary gland mesenchymal (eSGM) feeder cell layer formation. ( b ) Cell viability of eSGM cells cultured for 48 h under each condition ( n = 6). ( c ) mRNA expression level of EGF and FGF7 in eSGM cells cultured for 48 h under each condition ( n = 3). ( d ) Immunofluorescence images of mesenchymal CD44s at 4 h after seeding. Scale bar = 50 µm ( n = 3). ( e ) Quantification of CD44-clustered eSGM cells within a defined visual field area ( n = 3). ( f ) Immunofluorescence images of mesenchymal CD44s and EGF in eSGM cells cultured for 48 h on control or HA–CA-coated surfaces. Scale bar = 50 µm ( n = 3). ( g ) Quantification of mesenchymal EGF expression in eSGM cells. Pixel area where EGF intensity is higher than 100 AU are measured and divided by the defined visual field area ( n = 4). ( h ) Linear correlation is plotted between signal intensities of CD44 and EGF in the immunofluorescence images. R 2 and p -values are noted in the graph. Data are expressed as average ± SEM ( b,c,g) or median with interquartile range ( e). * p < 0.05, *** p < 0.005, **** p < 0.001, ns = non-significant ( p > 0.05) by unpaired t -test ( g), one-way ANOVA with Dunnett’s tests ( b,c), and Kruskal–Wallis ANOVA with non-parametric Dunnett’s test ( e).

Article Snippet: The permeabilized eSMGs or mesenchymal cells were then blocked by immersion in PBSX containing 10% normal donkey serum (NDS; Sigma-Aldrich) and 1% mouse-on-mouse (MOM) blocking reagent (Vector Laboratories, MKB-2213-1; Burlingame, CA, USA) at RT for 3 h. After the blocking step, eSMGs or mesenchymal cells were incubated with PBSX containing primary antibodies (1:100) and 3% NDS at 4 °C for 12 h. The primary antibodies used in the procedure were as follows: goat polyclonal anti-c-kit antibody (R&D System, AF1356; Minneapolis, MN, USA), rat monoclonal anti-CD44 antibody (Abcam, ab157107; Cambridge, UK), mouse monoclonal anti-TUJ1 (R&D System, MAB1195), rabbit polyclonal anti-E-cadherin (Cell Signaling Technology, 3195S; Beverly, MA, USA), mouse monoclonal anti-EGF (Invitrogen, MA5-15606), and rabbit polyclonal anti-FGF7 (Invitrogen, PA5-49715; Carlsbad, CA, USA).

Techniques: Cell Culture, Expressing, Immunofluorescence

Journal: PeerJ

Article Title: Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy

doi: 10.7717/peerj.4306

Figure Lengend Snippet: The information of primers in RT-PCR experiment.

Article Snippet: Then, the cover slides were rinsed with PBS and incubated with 5% goat serum in PBS at room temperature for 1 h. After that, slides were incubated with a rabbit anti-FGF7 antibody (1:100; Boster, Wuhan, China) or a rabbit anti-α-SMA antibody (1:200; Bioss, Beijing, China) at 4 °C overnight and subsequently with appropriate secondary antibodies (1:500; ZSGB-bio, Beijing, China).

Techniques: Sequencing

Journal: Biochemical and biophysical research communications

Article Title: FGF7 supports hematopoietic stem and progenitor cells and niche-dependent myeloblastoma cells via autocrine action on bone marrow stromal cells in vitro.

doi: 10.1016/j.bbrc.2013.09.044

Figure Lengend Snippet: Fig. 3. Fgfr2IIIb is expressed in MEFs and BM stromal cells, but not in BM hematopoietic cells. (A) Quantitative PCR. Fgfr1, 2IIIb, 2c, 3, and 4 mRNAs were expressed in BM stromal cells (MS-5 and OP-9), osteoblasts (MC3T3-E1), and MEFs. BM hematopoietic cells expressed Fgfr1, 2c, 3, and 4 mRNAs but not Fgfr2IIIb mRNA. The values (mean ± SD of a representative experiment performed in triplicate) are plotted as the fold increase versus the value in Med1+/+ MEFs. (B) Western blot. FGF7 receptor FGFR2IIIb was expressed comparably in BM stromal cells, osteoblasts, and MEFs.

Article Snippet: The next day, 1 106 BM cells, harvested from the femurs of congenic wild-type mice, were added to each well and cultured in MyeloCult M5300 (Stem Cell Technologies, Canada) and 10 U/mL heparin in the absence or presence of various amounts of recombinant human (rh) FGF7 (R&D Systems), 0.2 lg/mL anti-mouse (m) FGF7 rabbit polyclonal IgG (H-73: sc-7882; Santa Cruz), or normal rabbit IgG (Sigma) at 33 C. For long-term culture, half of the medium was replaced with fresh medium each week.

Techniques: Real-time Polymerase Chain Reaction, Western Blot